The effect of enzyme concentration on enzyme activity coursework

Most enzymes in the human body have an Optimum Temperature of around This will decrease the rate of reaction. This is shown when oxygen stops being produced and the same results are recorded five times. Afterward, different solutions are filled in each test tube: I believe that the data also shows strong positive correlation, and there are few outliers, which shows that my results are accurate.

This is because the molecules of both substrate and enzyme have more energy, therefore they collide more often and produce more heat energy. A beaker is filled? Enzymes are proteins that speed up the rate of chemical reactions. Conversely, this activity had a stop point where activity ceased suddenly Raven Also, I noticed if the barrel was wet, the syringe often got stuck for a short time before it recorded the volumes of gas.

I have drawn a line of best fit to The effect of enzyme concentration on enzyme activity coursework illustrate this trend. Firstly, more oxygen dissolves in water at low temperatures than at high temperatures, meaning that for the reactions involving low concentrations, more oxygen would have dissolved than in the higher concentrations because of the decreased amount of heat energy given off.

However, I also believed that if I halved the concentration then the rate of reaction volume of oxygen produced would also be halved, and so the rate would be proportional to the concentration. Breaking bonds within the enzyme will cause the Active Site to change shape.

As I have mentioned earlier, this decreases in steps of 3. Different enzymes have different Optimum pH values. For example, the enzyme Pepsin functions best at around pH2 and is found in the stomach, which contains Hydrochloric Acid pH2.

This would show that the reaction is a first order reaction. This should tell me whether the reaction is indeed a first-order reaction, or if a curve of best fit is required. Consistent Temperature Temperature is a major factor which affects the rate of reaction.

This heat energy is transferred to the environment. Temperature variations were observed and recorded. In order to do this, we added different levels of pH into different test tubes with the enzyme sucroseand substrate sucroseand we then inverted the tube. I tried to keep this constant by making sure I swirled the conical flask evenly.

Factors affecting Enzyme Activity Factors affecting Enzyme Activity The activity of an Enzyme is affected by its environmental conditions. Four test tubes are labeled 1A-4A along with four additional tubes labeled 1B-4B.

Substrate concentration testing showed that as when the substrate increased, the activity of the enzyme also increased.

I decided on the gas syringe method because, as I explained in my section on preliminary work, it measured the volume of gas directly and minimised the volume of oxygen which could potentially dissolve in water. This is shown in the table with all the results I have gained Fig. Controlling these factors in a cell is one way that an organism regulates its enzyme activity and so its Metabolism.

Cells control the production of these enzymes, and if the breakdown of these enzymes exceeds synthesis, the rate slows down. Lastly, part three of this experiment strives to understand the effects of pH on the rate of enzyme activity.

Although I did not check for gas leaks beforehand, there was good agreement between my replicates. The highest rate of reaction, known as the Initial Reaction Rate is the maximum reaction rate for an enzyme in an experimental situation. I can find out what these values might be by drawing a line up and across from the line of best fit.

I believe that my results were also relatively reliable because as the concentration decreased the volume of oxygen produced also decreased. Each piece of apparatus has an apparatus error with an upper and lower limit.

The optimal temperature is the highest rate of reaction. This means that having the same surface area of yeast in each reaction is very important in ensuring a fair test because the number of molecules exposed to collisions must be the same.

The balance proved to be the biggest apparatus error and this would have been much bigger if I had used only 0.Determination of the effect of enzyme concentration on catalysis using starch an amylase.

Factors affecting Enzyme Activity

INTRODUCTION. Enzymes are said to be catalytic proteins which increases the rate of a chemical reaction without being altered in the process of that reaction. [1] A substrate is a substance which an enzyme acts upon.

Introduction to Enzymes. In order to study the effect of increasing the enzyme concentration upon the reaction rate, the substrate must be present in an excess amount; i.e., the reaction must be independent of the substrate concentration.

To measure enzyme activity ideally, the measurements must be made in that portion of the curve. Apr 30,  · Investigate the effect of substrate concentration on the rate of activity of the enzyme catalase. Hypothesis I believe that as the concentration of the hydrogen peroxide (substrate) decreases, the rate of reaction will decrease as mi-centre.coms: 5.

Substrate concentration testing showed that as when the substrate increased, the activity of the enzyme also increased.

Introduction to Enzymes

Keyword: enzyme, substrate, sucrose, sucrose The Effect of Concentration, pH and Temperature on Enzyme Activity Introduction In this lab we explore enzyme activity and how it can be affected by changes to its environment. The Effect Of Substrate Concentration On The Activity Of The Enzyme Catalase A Level Biology Project Aims This is an experiment to examine how the concentration of the substrate hydrogen peroxide affects the rate of reaction of the enzyme catalase.

The Effects of Enzyme Concentration on the Activity of Amylase To investigate the effect of Amylase concentration on its activity.

the relative activity of Amylase is found by noting the time taken for the starch substrate to be broken down, that is, when it is no longer gives a blue-black colour when tested with iodine solution.

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The effect of enzyme concentration on enzyme activity coursework
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